IMPORTANT: Read the Prep & Injection Guide for proper reconstitution, syringe sizing, and injection protocols. Mistakes here can compromise your research.
The “Wolverine” blend combines two of the most widely researched regenerative peptides — BPC-157 and TB-500 (Thymosin Beta-4 fragment) — in a single vial. BPC-157 is a stable gastric pentadecapeptide studied for angiogenesis, tendon-to-bone healing, gut repair, and anti-inflammatory activity. TB-500 is studied for cell migration, actin regulation, flexibility of tissue, and reduction of scar formation. Together the pair is researched for synergistic healing of muscle, tendon, ligament, and connective tissue — the reason the combination earned its “Wolverine” nickname.
— 10 mg Blend Vial —
Reconstitute: Add 2.0 mL (200 units) bacteriostatic water.
Administration: Subcutaneous or intramuscular injection (may rotate between the two).
Timing: May be taken at any time of day; dosing does not require fasting.
Storage: Lyophilized: freeze at −20 °C; reconstituted: refrigerate at 2–8 °C, avoid freeze-thaw cycles.
Schedule: Once-daily injections for an 8-week cycle.
Cycle Length: 8 weeks, followed by a 4–8 week washout period.
Goal: Support soft-tissue, tendon, and ligament healing based on preclinical evidence.
Frequency: Inject once daily (subcutaneous or intramuscular).
Standard Protocol
| Phase | Dose | Syringe (U-100) |
|---|---|---|
| Weeks 1–2 | 600 mcg (0.6 mg) | 6 units (0.06 mL) |
| Weeks 3–8 | 800 mcg (0.8 mg) | 8 units (0.08 mL) |
Maximum Protocol
| Phase | Dose | Syringe (U-100) |
|---|---|---|
| Weeks 1–2 | 1,000 mcg (1.0 mg) | 10 units (0.10 mL) |
| Weeks 3–8 | 600 mcg (0.6 mg) | 6 units (0.06 mL) |
Note: Injection site can influence systemic bioavailability, with abdominal (stomach) injection generally offering the best overall absorption.
- Synergistic healing of muscle, tendon, ligament, and connective tissue injuries.
- Accelerates wound healing through angiogenesis (formation of new blood vessels).
- Reduces systemic and localized inflammation.
- Supports tendon-to-bone healing and ligament repair.
- Promotes cell migration to injury sites (TB-500’s actin-regulating mechanism).
- May reduce scar tissue formation and improve tissue flexibility.
- Supports gut-lining repair and gastrointestinal health (BPC-157).
- Protects against free radical damage with antioxidant activity.
- May improve recovery time from training and injury.
- Studied for joint comfort and improved mobility.
- Cardiovascular tissue support via improved blood flow and oxygenation.
- Tendon & ligament injuries (e.g. Achilles, rotator cuff)
- Muscle strains, tears, and crush injuries
- Joint injuries and chronic joint pain
- Post-surgery recovery
- Chronic inflammatory conditions
- Arthritis / Osteoarthritis
- Nerve injuries
- Ulcers and inflammatory bowel conditions (BPC-157 component)
- Slow-healing wounds
While side effects are not common, you may experience:
- Nausea
- Headaches
- Dizziness
- Fatigue or lethargy
- Gas/bloating or appetite changes
- Irritation or redness at the injection site
- Do not use when pregnant or nursing.
- Do not use with active cancer — both compounds promote angiogenesis and cell migration.
- Do not take if you have chronic kidney disease; use caution with liver disease.
- Exercise caution with protein allergies.
- If you have an autoimmune disorder, it may be worsened since BPC-157 stimulates the immune system.
- Exercise caution with heart conditions, as BPC-157 may cause a rise in blood pressure.
Either subcutaneous or intramuscular injection may be used for this blend, and you may rotate between the two. Injection region affects systemic bioavailability — the stomach generally offers the best absorption overall.
Subcutaneous
Pro’s: Provides systemic benefits by entering the bloodstream and circulating throughout the body; good for both general healing and healing multiple injuries.
Con’s: Absorption is slower than IM injections.
Intramuscular
Pro’s: Faster absorption and more noticeable effects. Suitable for acute injuries, post-surgery recovery. Recommended for targeted pain relief near the affected area.
Con’s: Some may consider IM injections to be more difficult and/or more painful.
The Wolverine blend is itself a stack of BPC-157 and TB-500. Common research pairings to extend it include:
- Ipamorelin – For stimulating GH release and promoting full-body repair
- CJC No DAC – Enhances GH pulsatility alongside Ipamorelin
- Sermorelin – A GHRH analog to further support repair and recovery
- GHK-Cu – Known for wound healing and skin repair
- AOD-9604 – For combining fat reduction with accelerated healing
PubMed-linked citations for verification. Many studies are animal/in-vitro.
- Gastric pentadecapeptide BPC 157 accelerates healing of transected rat Achilles tendon. J Orthop Res. 2003;21(6):976-82. PMID: 12740856
- Therapy with gastric pentadecapeptide BPC 157 for various muscle and tendon pathologies. Eur J Pharmacol. 2005;510(1-2):113-7. PMID: 16024785
- Pentadecapeptide BPC 157 enhances the growth hormone receptor expression in tendon fibroblasts. Growth Horm IGF Res. 2014;24(1):22-8. PMID: 19770133
- Thymosin beta 4 accelerates wound healing. J Invest Dermatol. 1999;113(3):364-8. PMID: 10469335
- Thymosin beta4 promotes cardiac repair and cardiomyocyte survival. Nature. 2004;432(7016):466-72. PMID: 15565145
- Thymosin β4: a multi-functional regenerative peptide. Expert Opin Biol Ther. 2015;15 Suppl 1:S139-45. PMID: 26096726
- Pentadecapeptide BPC 157 — a therapy for various conditions. J Pharmacol Sci. 2018;138(1):1-8. PMID: 29522634
Research Use Only. All information on this page is for educational purposes only and is not medical advice. PepSherpa does not sell peptides. Consult a licensed healthcare provider before making any health decisions. Many of the studies cited are preclinical (animal/in-vitro).